Primer
Part:BBa_K5103006:Experience
Designed by: Nikoo Mansourian Group: iGEM24_Guelph (2024-09-22)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K5103006
This part was used to introduce a HindIII cut site to the 3' end of Cry8Da (part: BBa_K5103000), we had no problems with the PCR protocol and successfully optimized a PCR protocol for both Forward and Reverse primers (Parts: BBa_K5103007 & BBa_K5103006).
PCR protocol:
Denaturation: 95°C - 30 sec
Annealing: 51°C - 30 sec
Extention: 72°C - 10 min
Repeat 34X
User Reviews
UNIQdf209c1d4718e859-partinfo-00000000-QINU UNIQdf209c1d4718e859-partinfo-00000001-QINU